Anti-BBP Antibody

This polyclonal antibody targets the BBP splicing factor (also known as SF1, branchpoint-binding protein), a protein that recognizes the branchpoint sequence during early spliceosome assembly on pre-mRNA. Raised in rabbits and supplied as an unconjugated IgG preparation at 1 mg/ml, this reagent is calibrated for Western blot workflows and has been validated on human and mouse samples. The product is buffered in PBS containing 0.1% sodium azide and 50% glycerol to support reproducible handling under refrigerated storage, and ships in a 100 µl vial sized for routine immunoblotting panels without frequent freeze-thaw cycling.

Researchers investigating U2-type intron recognition, RNA processing, and gene expression regulation rely on this reagent to probe endogenous BBP levels in cell lysates. The polyclonal nature of the antibody improves the chance of detecting the protein across evolutionary-conserved epitopes shared between the human and mouse orthologs, useful when comparing experimental systems. Sodium azide is included to inhibit microbial growth during repeated access from a refrigerated vial, while glycerol prevents freezing of the solution, so the antibody can be dispensed directly into lysis buffer for downstream Western blot assays.

Each lot is quality controlled at Antibodies.com and supports use in standard immunoblotting. The unconjugated format provides downstream flexibility, allowing the researcher to pair the primary antibody with HRP-, fluorophore-, or biotin-conjugated secondary reagents depending on the detection platform chosen.