Anti-BBC3 Antibody

BBC3 (also known as PUMA, p53 upregulated modulator of apoptosis) is a BH3-only member of the Bcl-2 family that drives mitochondrial apoptosis in response to DNA damage, oncogenic stress, and cytotoxic insult. This polyclonal reagent from Antibodies.com is raised in rabbit and supplied as an unconjugated IgG preparation of 200 µL, validated for western blot detection of endogenous BBC3 in human, mouse, and rat samples.

The antibody binds the full-length BBC3 protein, which resolves as an approximately 20–23 kDa band under reducing SDS-PAGE conditions. Researchers apply it to study p53-dependent apoptotic signaling, BH3-only contributions to mitochondrial outer membrane permeabilization, and apoptotic priming in cancer cell lines. Comparable polyclonal reagents have been used to track BBC3 induction after genotoxic stress and to dissect its role in tissue injury models across rodent systems.

Because BBC3 sits at the convergence point of p53 signaling and intrinsic apoptosis, this antibody supports investigations in DNA damage response, tumor suppressor biology, neurodegeneration, ischemia-reperfusion injury, and chemotherapy-induced cell death. Aliquot the antibody upon receipt, store refrigerated at 4 °C, and avoid repeated freeze-thaw cycles to preserve reagent performance across repeated western blot experiments.