Anti-Bax (S163) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically targets the Bax protein, a pro-apoptotic member of the Bcl-2 family that drives the intrinsic mitochondrial pathway of programmed cell death. Supplied at 1 mg/ml in phosphate buffered saline (PBS) at approximately pH 7.2 with 0.05% sodium azide as a preservative, the reagent is unconjugated and provided as a 50 µl volume ready for multiple western blot and immunofluorescence experiments. Validated for WB and IF workflows, the antibody recognizes endogenous Bax and cross-reacts with human, mouse, and rat samples, supporting apoptosis research across standard mammalian model systems. As a polyclonal reagent raised in rabbit, it binds multiple epitopes on the Bax target, increasing sensitivity for detecting native protein levels in cell lysates and tissue extracts. Refrigerate upon arrival; for long-term use, aliquot the solution and freeze to limit repeated freeze-thaw cycles of the azide-containing buffer. Researchers investigating cytochrome c release, mitochondrial outer membrane permeabilization, p53-dependent signaling, or chemotherapeutic response pathways can apply this reagent to confirm Bax expression and subcellular translocation patterns in fixed cells and denatured protein preparations.