Anti-Bax Antibody

Raised in rabbit hosts against the Bcl-2-associated X protein, this polyclonal antibody from Antibodies.com targets Bax, a pro-apoptotic Bcl-2 family member that controls mitochondrial outer membrane permeabilization and cytochrome c release during intrinsic programmed cell death. Supplied as 100 µl of unconjugated antiserum, the reagent supports western blot, immunohistochemistry, immunofluorescence, and ELISA workflows across human, mouse, and rat samples.

Because Bax sits at the convergence point of cellular stress, DNA damage, and growth-factor withdrawal signaling, this antibody is widely deployed in cancer biology, neurodegeneration, and cardiotoxicity research programs where investigators need to track apoptotic commitment at the mitochondrial checkpoint. Its polyclonal format recognizes multiple epitopes across the Bax sequence, which helps when probing samples with variable expression or post-translational modification, including the conformational activation step Bax undergoes when translocating from cytosol to mitochondrial membrane.

For short-term use, refrigerate the vial; for extended storage, aliquot and freeze to limit repeated freeze-thaw exposure and preserve antibody titer. Optimal working dilution should be determined empirically per application, since immunofluorescence and immunohistochemistry protocols generally require substantially higher antibody concentrations than standard western blotting conditions.