Anti-Bax Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against the pro-apoptotic Bcl-2 family member Bax and is supplied as an unconjugated, ready-to-use liquid at a concentration of 1 mg/ml. The antibody is provided in a 50 µl aliquot formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide as preservative, and 50% glycerol as cryoprotectant, and should be stored at –20 °C. Validated applications include Western blot (WB) and immunohistochemistry (IHC), with reactivity confirmed against human, mouse, and rat samples, making it suitable for cross-species studies of apoptosis regulation. The immunogen corresponds to recombinant human Bax protein, and the antibody detects endogenous levels of total Bax. As a polyclonal reagent of rabbit origin, the immunoglobulin class is IgG, allowing straightforward pairing with the wide range of secondary antibodies available for chromogenic, fluorescent, or chemiluminescent detection systems.