Anti-BAX Antibody

BAX (BCL-2-associated X protein) is a pro-apoptotic member of the BCL-2 family that governs the mitochondrial intrinsic pathway of programmed cell death. Upon cellular stress, BAX translocates from the cytosol to the outer mitochondrial membrane, where it oligomerizes and triggers cytochrome c release, caspase activation, and commitment to apoptosis. Dysregulation of BAX is a recurring theme in cancer biology, ischemic injury research, and neurodegenerative disease studies, making it a widely targeted antigen across apoptosis-focused laboratories.

From Antibodies.com, this rabbit polyclonal anti-BAX antibody is supplied as 100 µl at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3. The unconjugated reagent is validated for western blot and detects endogenous BAX in samples from human, mouse, and rat, supporting cross-species work in cell lysates and tissue homogenates. The expected band appears near the predicted 21 kDa region under standard reducing SDS-PAGE conditions, where the rabbit host provides strong secondary antibody compatibility.

Recommended storage is at -20 °C in single-use aliquots to limit freeze-thaw cycles; the sodium azide in the formulation acts as a microbial preservative for routine benchtop use. Allow the vial to equilibrate before opening, then mix gently without vortexing to preserve antibody activity. For western blot, dilute into TBS-T or PBS-T with 5% non-fat milk or BSA during blocking and incubation, and pair with an appropriate anti-rabbit HRP or fluorescent secondary. Researchers studying chemotherapy response, neurodegeneration, and mitochondrial biology routinely use this reagent to assess BAX expression and translocation as a marker of apoptotic commitment.