Anti-Bax (Ab-167) Antibody

Manufactured by Antibodies.com, this Rabbit polyclonal antibody targets the pro-apoptotic Bax protein and is raised against a synthetic non-phosphopeptide derived from human Bax surrounding the threonine 167 phosphorylation site (F-G-T(p)-P-T). The antibody is supplied at a concentration of 1 mg/ml in a 50 µl volume, formulated as Rabbit IgG in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. It is unconjugated and has been affinity-purified from rabbit antiserum by epitope-specific affinity chromatography, ensuring selective recognition of endogenous levels of total Bax. Validated for Western blot (WB) and immunofluorescence (IF) applications, the reagent has been confirmed to react with both Human and Mouse samples, making it a versatile choice for apoptosis research across these species. Recommended storage is at –20 °C, and the product is intended for research use only, not for diagnostic or therapeutic applications. A representative Western blot image using extracts from HepG2 cells is provided on the supplier page to support application performance. With reactivity to two common research species, a well-characterised immunogen, and a flexible buffer formulation compatible with standard immunoassays, this polyclonal reagent is suited to studies investigating Bax expression, mitochondrial apoptotic pathways, and related cellular stress responses.