Anti-Bak Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes Bak (BCL2 antagonist/killer 1), a pro-apoptotic BCL-2 family member that localizes to the outer mitochondrial membrane and drives the intrinsic apoptosis pathway through mitochondrial outer membrane permeabilization. Supplied as an unconjugated reagent in a 100 µl volume, the antibody is recommended for Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) applications on human samples in research use only investigations of programmed cell death and mitochondrial signaling.

As a polyclonal reagent raised in rabbit, the antibody binds multiple epitopes across the Bak protein, supporting detection in both denatured Western Blot lysates and natively folded IHC or IF preparations. It is suited to studies of cancer therapeutics engaging the BCL-2 rheostat, and the interplay between Bak and related family members such as Bax, Bcl-2, Bcl-xL, and Mcl-1. The unconjugated format provides flexibility for downstream detection, pairing with HRP-, biotin-, or fluorophore-conjugated secondary antibodies matched to the chosen assay platform.

For storage, the antibody should be refrigerated at 4 °C for short-term use, with long-term stability maintained by freezing aliquots at -20 °C; repeated freeze-thaw cycles should be minimized to preserve binding activity. Researchers should consult the product data sheet for recommended starting dilutions across WB, IHC, and IF workflows.