Anti-Bak Antibody

The Bak protein (BCL2 antagonist/killer 1) is a pro-apoptotic BCL-2 family effector that, together with Bax, oligomerizes to permeabilize the mitochondrial outer membrane during intrinsic apoptosis. This unconjugated recombinant rabbit monoclonal antibody from Antibodies.com is raised against Bak and validated for detection of the human protein across Western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and flow cytometry workflows.

Each vial contains 100 µl of antibody in a TBS buffer (pH 7.4) supplemented with 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative. The high glycerol content keeps the formulation liquid at working freezer temperatures, supporting storage at -20 degrees C in single use aliquots to limit freeze-thaw damage. Researchers in cancer biology, mitochondrial signaling, and programmed cell death use this reagent to monitor Bak expression and activation status in human cell lines and tissue specimens.