Anti-Bak Antibody

Supplied by Antibodies.com as a 50 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this rabbit polyclonal reagent targets Bak (BCL2 antagonist/killer 1), a pro-apoptotic Bcl-2 family member that localizes to the outer mitochondrial membrane and drives cytochrome c release during intrinsic apoptosis signaling. Investigators working on programmed cell death, mitochondrial pathway analysis, and tumor biology apply it to track apoptotic responses in cell-based and tissue-based model systems.

The unconjugated antibody is validated for western blot, immunohistochemistry, and immunocytochemistry across human, mouse, and rat samples, so endogenous Bak levels can be compared in lysates, fixed cells, and paraffin-embedded tissue sections from matched control and treatment groups. Polyclonal recognition of multiple epitopes improves tolerance for minor sequence variation between species and supports reproducible band detection on blots and clear cytoplasmic staining patterns in IHC.

Keep the vial frozen at -20°C in single-use working aliquots to limit freeze-thaw cycles; the glycerol cryoprotectant maintains activity for months under these storage conditions. Briefly centrifuge before opening, handle on ice, and return promptly to the freezer after each use to preserve titer for ongoing western blot and immunostaining experiments.