Anti-BAF53 Antibody

BAF53 (also known as ACTL6A or Arp4) is an actin-related subunit of the ATP-dependent SWI/SNF chromatin remodeling complex. It contributes to nucleosome repositioning and transcriptional regulation across development and disease, with documented involvement in neural progenitor maintenance, hematopoiesis, and cancers including medulloblastoma and acute myeloid leukemia.

This Antibodies.com polyclonal reagent was raised in rabbit against BAF53 and ships as a 50 µl vial at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol. The unconjugated format suits downstream labeling with fluorophores or horseradish peroxidase. Validated applications include Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation, with reactivity confirmed against human, mouse, and rat samples. Researchers mapping BAF complex occupancy or profiling SWI/SNF subunit composition in cancer models commonly deploy this reagent in ChIP experiments and IP-mass spectrometry workflows.

Store frozen at -20°C in the glycerol-containing buffer; aliquot on first thaw to limit freeze-thaw cycles and protect from light if labeled downstream.