Anti-BAF250B Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against BAF250B, the AT-rich interactive domain-containing protein ARID1B that anchors the mammalian SWI/SNF (BAF) ATP-dependent chromatin remodeling complex to chromatin. Supplied as 100 µL of unconjugated antiserum in a vial, the antibody is validated for western blot analysis of endogenous BAF250B and for immunofluorescence staining of fixed cells and tissue sections, supporting cell culture work and lysate-based studies of chromatin remodeling. The reagent recognizes the target in human and rat samples and is used by groups studying the BAF complex's contribution to neuronal differentiation, the developmental phenotypes linked to ARID1B haploinsufficiency such as Coffin-Siris syndrome, and the loss-of-function alterations reported in several solid tumors. Researchers apply it to follow changes in subunit expression across differentiation time courses and to compare BAF250B levels between patient-derived and control cells.