Anti-BAF250 Antibody

This rabbit polyclonal primary antibody detects BAF250, also known as ARID1A, in human cells. Supplied by Antibodies.com as an unconjugated rabbit IgG in a 100 µl liquid aliquot, it is intended for research use only and can be paired with a labeled secondary antibody or a fluorophore selected for the experimental design.

Validated for immunofluorescence (IF), the reagent is used to examine nuclear BAF250 distribution in fixed human specimens. It supports research on the SWI/SNF chromatin-remodeling complex, including comparisons of nuclear staining among human cell models and investigation of altered ARID1A expression in cancer biology. Because the antibody is unconjugated, researchers retain control over fluorophore selection and can combine it with compartmental or cell-state markers in multicolor imaging. The polyclonal format recognizes multiple epitopes on BAF250; appropriate positive, negative, and secondary-antibody controls should be included to confirm specificity.

Keep the vial refrigerated, protect it from light, and avoid repeated freeze-thaw cycles. Dilution, fixation, permeabilization, and incubation conditions may require optimization for each cell type and imaging protocol.