Anti-Bad (phospho-S91) Antibody

The Bcl-2-associated death promoter (Bad) is a BH3-only pro-apoptotic member of the Bcl-2 family whose activity is tightly controlled by phosphorylation at multiple serine residues. This rabbit polyclonal antibody from Antibodies.com specifically detects Bad when phosphorylated at serine 91 (phospho-S91), a regulatory site within the BH3 domain whose modification disrupts binding to anti-apoptotic Bcl-2 family partners and shifts cells away from apoptosis.

Supplied as 100 µl of an unconjugated reagent at 1 mg/ml in phosphate-buffered saline (pH approximately 7.2) with 0.05% sodium azide, the antibody is validated for Western blot (WB) and immunohistochemistry (IHC) applications and cross-reacts with Human, Mouse, and Rat samples. Researchers studying survival signaling downstream of kinases such as Akt/PKB rely on anti-phospho-S91 Bad detection to monitor pathway activation status in cell lysates and tissue sections.

Typical uses include tracking apoptosis regulation in cancer cell line models, characterizing survival responses in neuronal and cardiac ischemia-reperfusion systems, and assessing signaling through PI3K/Akt cascades in metabolic and immune research. Aliquot upon first use to minimize freeze-thaw cycles and store refrigerated; the format supports routine Western blotting and chromogenic IHC on formalin-fixed tissue.