Anti-Bad (phospho S112) Antibody

Phosphorylation of the Bcl-2-associated agonist of cell death at serine 112 is a hallmark of active pro-survival signaling, and Antibodies.com offers a rabbit polyclonal IgG reagent raised specifically against this post-translational modification. The unconjugated antibody is supplied in a 50 µl vial and is validated for Western blot and immunohistochemistry on human, mouse, rat, and monkey samples, giving laboratories a single reagent for cross-species mapping of the PI3K/Akt and RSK kinase cascades. Storage at -20 °C in working aliquots minimizes repeated freeze-thaw cycles and preserves phospho-epitope recognition, which is critical because the phospho-S112 mark is labile and can be lost during sample handling.

Because the S112 phospho-site generates a 14-3-3 docking motif that retains Bad in the cytosol, the antibody is widely adopted in studies of growth factor signaling, serum withdrawal, oxidative stress, and small-molecule kinase inhibitor treatment, where it is used to distinguish the inactive, phosphorylated pool from the dephosphorylated, pro-apoptotic form that drives cytochrome c release and caspase-9 activation. The polyclonal format provides a robust signal for tracking dynamic changes in phosphorylation after neurotrophin stimulation, ischemic preconditioning, or chemotherapeutic challenge, and the unconjugated IgG is compatible with the secondary antibody detection chemistries of the researcher's own choice.