Anti-Bad (phospho S112) Antibody

This phospho-state-specific primary antibody detects endogenous Bad only when serine 112 is phosphorylated, enabling researchers to distinguish this regulatory modification from total Bad protein. The reagent is useful for investigating pro-apoptotic signaling, survival-pathway activity, cellular stress responses, and treatment-induced pathway changes.

Antibodies.com supplies 100 µl of unconjugated rabbit polyclonal IgG in a vial. The antibody is listed for Western blot and immunohistochemistry and is reactive with human, mouse, rat, and monkey samples. This broad species reactivity supports consistent analysis across cell lines, primary cultures, experimental animal models, and tissue-based research.

Typical research applications

• Western blot analysis of Bad phosphorylation in lysates collected after pathway stimulation, inhibition, or time-course treatment.
• Immunohistochemical localization of phosphorylated Bad in tissue sections from cancer, neuroscience, or metabolic disease models.
• Cross-species comparison of survival and apoptosis signaling in human specimens and relevant animal systems.
• Evaluation of kinase-pathway engagement or resistance mechanisms in response to experimental compounds.

For meaningful interpretation, researchers should compare results with appropriate total-protein, loading, and phosphorylation controls. The unconjugated format allows compatibility with common secondary-antibody detection systems. This product is for research use only and is not intended for diagnostic, therapeutic, or clinical procedures.