Anti-Bad (phospho S112) Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against Bad (Bcl-2-associated death promoter) phosphorylated at serine 112, a regulatory site that controls the protein's pro-apoptotic function within the Bcl-2 family. Phosphorylation at S112 by kinases such as Akt/PKB, PKA, and p90RSK creates a docking site for 14-3-3 scaffolding proteins, sequestering Bad in the cytosol and connecting this epitope to research on cell survival, growth factor signaling, and mitochondrial apoptosis pathways. The reagent is validated for Western blot detection in human, mouse, and rat samples and is supplied as 200 µl of unconjugated IgG, suitable for chemiluminescent or chromogenic detection workflows. Researchers studying PI3K/Akt pathway activity, cancer cell survival, or neuronal apoptosis models can use this reagent to monitor dynamic S112 phosphorylation in response to growth factors, cytokines, or cellular stress. Aliquot upon arrival and store at -20°C to preserve phospho-specific binding activity and minimize freeze-thaw cycles. For research use only.