Anti-BAD Antibody

Raised in rabbit, this unconjugated polyclonal antibody recognizes BAD (BCL2-associated agonist of cell death), a BH3-only member of the Bcl-2 family that promotes apoptosis by sequestering anti-apoptotic partners such as Bcl-2 and Bcl-xL at the mitochondrial outer membrane. Phosphorylation of BAD at conserved serine residues, including Ser112, Ser136, and Ser155, controls its binding affinity and links the protein to upstream kinase signaling through the Akt and MAPK pathways.

Validated for Western blot and immunohistochemistry workflows across human, mouse, and rat samples, the reagent is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2. The polyclonal format provides recognition of multiple epitopes on the target, which is useful when probing phosphorylation states, isoform expression, or partial degradation products in cell lysates and tissue sections.

Researchers studying intrinsic apoptotic pathways, cancer cell survival, ischemic injury, neuronal cell death, or insulin and glucose signaling will find this reagent relevant, because BAD integrates growth factor and survival cues downstream of kinases such as Akt. The unconjugated format leaves the antibody free for custom labeling or for use with secondary detection reagents matched to the experimental system.

Provided as a 100 µL liquid aliquot from Antibodies.com, the antibody should be handled with standard cold-chain precautions and protected from repeated freeze-thaw cycles to preserve binding activity. The sodium azide in the buffer makes the product compatible with fixed tissue and lysate-based protocols but unsuitable for live-cell applications.