Anti-Bacillus Antibody

This polyclonal preparation from Antibodies.com is a rabbit-derived primary reagent raised against Bacillus antigens and supplied as a purified IgG fraction in a 500 µl volume. Because it is unconjugated, the antibody can be paired with most enzyme- or fluorophore-labeled secondary reagents, giving laboratories flexibility to configure either chromogenic or fluorescent readouts.

The reagent is suited to ELISA, western blot (WB), immunohistochemistry (IHC), and immunofluorescence assay (IFA), covering the major workflows used to detect bacterial antigens in culture, tissue sections, and protein blots. The polyclonal format provides recognition of multiple epitopes on the target organism, which is useful when working with strain variation or with denatured proteins transferred to nitrocellulose or PVDF membranes following SDS-PAGE.

For best results, users typically aliquot the IgG fraction upon first thaw to limit freeze-thaw cycles and protect binding activity across repeated experiments. Working dilutions should be titrated against the chosen secondary detection system and sample type, since fixation, embedding, and block conditions vary between IHC, IFA, and WB protocols.