Anti-B3GNT5 Antibody

B3GNT5 is a Golgi-localized beta-1,3-N-acetylglucosaminyltransferase that initiates the type 2 chain on glycoproteins and supplies the poly-N-acetyllactosamine substrate extended downstream by B3GNT8. Functional studies link this enzyme to Notch1 receptor glycosylation and trafficking, so immunoblots probing Notch pathway perturbations often rely on a clean antibody against the endogenous transferase. Antibodies.com provides a rabbit polyclonal IgG raised against B3GNT5, supplied as 100 µl of unconjugated antibody validated for Western blot detection across human, mouse, and rat samples. Researchers studying glycosylation phenotypes, comparative expression between tumor and matched-normal tissue, or receptor-level readouts after manipulation of the LacNAc biosynthesis pathway can apply this reagent to whole-cell and primary-tissue lysates under standard reducing SDS-PAGE conditions. The unconjugated format leaves the choice of secondary open, which is useful when probing B3GNT5 alongside other glycosyltransferases in the same blot.