Anti-B2M Antibody

Raised in rabbit as an unconjugated polyclonal reagent, this anti-B2M antibody recognizes the approximately 11.8 kDa beta-2-microglobulin light chain that pairs non-covalently with MHC class I heavy chains on the surface of nucleated cells. Researchers apply it to western blot analysis of cell lysates, immunohistochemistry on formalin-fixed tissue sections for evaluating MHC I surface expression, and immunofluorescence imaging of cell-surface staining patterns.

From Antibodies.com, the reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, and the 100 µl vial supports many individual staining or blotting experiments. Documented reactivity covers human and mouse samples, making the antibody useful for comparative studies of patient-derived material alongside murine xenograft or knockout models, including work on MHC class I antigen presentation, immune evasion by tumor cells that downregulate surface B2M, and renal tubular protein handling, since beta-2-microglobulin filtered at the glomerulus is normally reabsorbed by proximal tubules. The product is intended for research use only.

Keep refrigerated after receipt; the glycerol component guards against freeze-thaw damage for short-term storage. Aliquotting is recommended to limit repeated freeze-thaw cycles when the vial is consumed gradually across weeks or months of work.