Anti-B-raf Antibody [K21-F]

A FITC-conjugated rabbit monoclonal antibody from Antibodies.com that detects BRAF (B-raf) protein in human cells via flow cytometry. The K21-F clone recognizes the native BRAF kinase, with reactivity validated specifically against human samples. The reagent is supplied as a 100T liquid format sized for routine immunophenotyping panels where BRAF expression is being characterized in hematopoietic and tumor-derived cell populations.

BRAF is a serine/threonine protein kinase that operates within the RAS-RAF-MEK-ERK signaling axis, transmitting mitogenic cues from activated RAS GTPases through MEK and ERK and controlling proliferation, differentiation, and survival. Aberrant BRAF activity, most notably driven by the V600E mutation, is a hallmark of melanoma, papillary thyroid carcinoma, hairy cell leukemia, and a substantial fraction of colorectal cancers, making BRAF a heavily studied target in both basic signaling research and clinical biomarker development. The FITC label supports co-staining with other directly conjugated antibodies in multicolor flow cytometry panels, enabling simultaneous interrogation of BRAF alongside lineage, activation-state, and viability markers without secondary antibody steps.