Anti-B-RAF (Ab-446) Antibody

Offered by Antibodies.com, this affinity-purified reagent is a rabbit polyclonal antibody raised against a synthetic non-phosphopeptide derived from human B-RAF surrounding the serine 446 phosphorylation site (R-D-S(p)-S-D), allowing it to recognise endogenous levels of total B-RAF protein in Western blot and immunohistochemistry workflows. The antiserum is produced in rabbit and purified by affinity-chromatography using the epitope-specific immunogen, then supplied as an unconjugated IgG at 1.0 mg/mL in phosphate-buffered saline (pH 7.4, 150 mM NaCl) supplemented with 0.02% sodium azide and 50% glycerol; this 50 µL format is part of a two-size range that also includes a 100 µL option. Validation data published by Antibodies.com list Western blot (WB) and immunohistochemistry (IHC) as the tested applications, with reactivity restricted to human samples, making the reagent well suited for studies of the B-Raf proto-oncogene serine/threonine-protein kinase in research settings. Shipments are dispatched cold from St. Louis, MO with a typical lead time of 6–9 business days, and the product is intended for research use only.