Anti-B-RAF (Ab-446) Antibody

This rabbit polyclonal antibody from Antibodies.com targets B-RAF, a serine/threonine kinase central to the MAPK/ERK signaling cascade and a focus of intensive oncology research because activating mutations such as V600E drive melanoma, colorectal carcinoma, and papillary thyroid carcinoma. The reagent is supplied as 100 µl of polyclonal sera, is unconjugated, and is validated for Western blot and immunohistochemistry on human tissue and cell lysates.

The Ab-446 epitope designation indicates that the immunogen was a synthetic peptide spanning residue 446 of the human B-RAF protein, within the region linking the CR2 conserved regulatory motif to the catalytic kinase domain. Because polyclonal sera recognize multiple epitopes within the immunizing peptide, the antibody yields a strong band near the expected molecular weight of full-length B-RAF in Western blot lysates and produces cytoplasmic staining patterns in formalin-fixed, paraffin-embedded sections prepared for immunohistochemistry.

Recommended handling includes aliquoting the vial upon first thaw to limit freeze-thaw cycles, storage at -20°C for long-term stability, and brief equilibration to room temperature before returning it to the freezer. The unconjugated format lets researchers pair this primary antibody with their own choice of HRP-, fluorophore-, or biotin-conjugated secondary reagents for chemiluminescent, fluorescence, or chromogenic detection workflows.