Anti-b-Catenin (Ab-41 / 45) Antibody

Antibodies.com offers a Rabbit polyclonal antibody raised against b-Catenin, designed to recognise the region around amino acids 39–43 / 43–47 of the human protein. The unconjugated reagent is validated for Western blotting (WB) and reacts with Human, Mouse and Rat samples.

According to the manufacturer, the antibody was generated by immunising rabbits with a synthetic peptide–KLH conjugate corresponding to the sequence A-T-T-T-A-P-S-L-S derived from Human b-Catenin, and was subsequently purified by affinity-chromatography using the epitope-specific peptide. It detects endogenous levels of total b-Catenin protein. The product is supplied at a concentration of 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol. Predicted molecular weight is reported as 92 kDa. The recommended Western blotting dilution range is 1:500–1:1000.

For handling, the antibody is shipped at 4°C; upon delivery it should be aliquoted and stored at −20°C, with freeze/thaw cycles avoided. The product is supported by 1 scientific validation image.