Anti-b-Catenin (Ab-37) Antibody

Beta-catenin (CTNNB1) is a multifunctional protein central to canonical Wnt signaling and adherens junction-mediated cell adhesion, and tracking its expression and post-translational state underpins studies of embryonic patterning, stem cell maintenance, epithelial–mesenchymal transition, and colorectal cancer biology.

This rabbit polyclonal reagent from Antibodies.com is raised against a defined epitope in the N-terminal region of human beta-catenin and is supplied as an unconjugated 50 µl liquid aliquot, ready for direct labeling or for use with standard secondary detection workflows.

Validated applications include Western blot for resolving the full-length ~92 kDa protein and downstream signaling readouts, immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, and immunofluorescence for subcellular localization at the membrane, cytoplasm, and nucleus. The antibody reacts with human, mouse, and rat samples, supporting comparative pathway studies across cell line panels, primary cultures, and rodent disease models without the need for separate species-specific reagents.

The polyclonal format provides multi-epitope coverage that can increase tolerance for minor degradation products often observed in crude lysates, while the unconjugated presentation leaves the choice of fluorophore, biotin, or HRP conjugate to the user. Recommended handling: aliquot upon first thaw to limit freeze-thaw cycling, keep refrigerated for active use, and freeze at –20 °C for longer-term storage; protect from light. Intended for research use only, intended for laboratory investigation of beta-catenin biology, Wnt pathway mapping, and antibody-based validation work.