Anti-ATPase beta3 (Na+ / K+) Antibody

This rabbit polyclonal antibody from Antibodies.com targets the beta-3 subunit of the Na+/K+ ATPase (ATP1B3), a regulatory subunit that assists the catalytic alpha subunit in achieving proper folding, maturation, and stable insertion into the plasma membrane. The beta-3 isoform is widely expressed in neuronal tissue, glia, and germ cells, and has attracted particular attention in oncology research where elevated ATP1B3 expression has been linked to glioblastoma cell invasion.

Supplied as 100 µl of unconjugated antiserum in a single vial, the reagent is validated for Western blot and immunohistochemistry on human samples. Host species is rabbit, and clonality is polyclonal, which is useful for researchers seeking broader epitope coverage when probing denatured lysates or fixed tissue sections. The unconjugated format allows investigators to pair the antibody with their own preferred secondary detection reagents, including HRP, biotin, or fluorescent conjugates.

Typical experimental use includes Western blot detection of the beta-3 subunit band in cell lysates, IHC localization of ATP1B3 in tissue sections, and immunofluorescence studies examining the polarized distribution of Na+/K+ ATPase in epithelial or neuronal preparations. The antibody should be stored refrigerated in working aliquots to limit freeze-thaw degradation of the polyclonal reagent.