Anti-ATP6V1B1 Antibody

The product targets ATP6V1B1, which encodes the B1 subunit of the vacuolar H+-ATPase (V-ATPase), a multi-subunit proton pump that acidifies intracellular organelles and extracellular compartments. ATP6V1B1 expression is largely restricted to specialized epithelia, most prominently the intercalated cells of the renal collecting duct and the stria vascularis of the inner ear, where loss-of-function mutations cause autosomal recessive distal renal tubular acidosis, often accompanied by progressive sensorineural hearing loss. Research applications for this antibody extend from studies of renal acid-base transport and cochlear ion homeostasis to emerging investigations of V-ATPase dysregulation in tumor microenvironments.

This rabbit polyclonal IgG reagent from Antibodies.com is unconjugated and supplied at 200 µl, validated for western blot, immunohistochemistry, and immunofluorescence on human samples. Researchers studying kidney physiology, hearing biology, or V-ATPase-related pathology can use it to probe B1 subunit localization and abundance in fixed tissue sections, cultured cells, and lysate preparations. Store refrigerated and protect from light; aliquot upon arrival and limit freeze-thaw cycles of the stock to preserve antibody activity across multiple experiments.