Anti-ATP6V0D1 Antibody

This rabbit polyclonal antibody targets ATP6V0D1, the d subunit of the vacuolar-type H⁺-ATPase (V-ATPase), a multi-subunit proton pump that acidifies intracellular organelles such as lysosomes, endosomes, and secretory vesicles. Distributed by Antibodies.com, the reagent is supplied as an unconjugated IgG in a 200 µl vial and has been validated for western blot detection of endogenous protein across human, mouse, and rat samples.

ATP6V0D1 is an integral membrane component of the V0 sector, which assembles with the cytosolic V1 sector to couple ATP hydrolysis with proton translocation. Because polyclonal reagents engage multiple epitopes on the target, the antibody supports consistent recognition of the subunit in lysates derived from cell culture and primary tissue. Laboratories investigating autophagy, endosomal maturation, neurotransmitter loading into synaptic vesicles, osteoclast-mediated bone resorption, and renal acid-base handling routinely profile V-ATPase subunit composition with reagents of this kind. The unconjugated format leaves the choice of secondary antibody, fluorophore, or enzyme reporter entirely to the user.

Recommended starting protocols begin with RIPA solubilization of cultured cells or freshly dissected tissue, separation by SDS-PAGE, transfer to nitrocellulose or PVDF, and probing in 5% non-fat milk or BSA blocking buffer. For storage, refrigerate the vial at 4 °C and aliquot on first use to limit freeze-thaw cycles; the antibody is intended for research use only.