Anti-ATP6AP2 Antibody

Offered through Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against ATP6AP2 (also known as the renin receptor / ATP6IP2 / M8-9), a type I transmembrane accessory subunit of the vacuolar H+-ATPase linked to renin–angiotensin signaling. Each 50 µl vial is supplied as a 1 mg/ml stock in PBS containing 0.1% sodium azide and 50% glycerol, and the antibody is unconjugated with an IgG isotype, making it suitable for use with secondary detection chemistries of the researcher's choice. The reagent has been validated for Western blot, immunohistochemistry and immunofluorescence workflows and is reactive with human samples, where it detects endogenous levels of total ATP6AP2 protein in lysates and fixed tissue. Polyclonal antibodies from Antibodies.com are typically affinity-purified against the immunogen and stored short term at 4 °C, with longer-term aliquots held at −20 °C to preserve activity; the included glycerol cryoprotectant and azide preservative allow the working stock to remain stable through repeated freeze–thaw cycles when handled aseptically. For Western blotting the antibody is commonly used in the 1:500–1:1000 range, while immunofluorescence on cultured cells is typically performed at 1:100–1:200 after appropriate fixation and permeabilization. Researchers working on V-ATPase assembly, intracellular pH regulation, or renin-receptor–mediated signal transduction in cardiovascular, renal or metabolic models will find this reagent a practical primary detection tool.