Anti-ATP6AP1 Antibody

Raised in rabbit against the vacuolar H+-ATPase accessory subunit ATP6AP1, this unconjugated polyclonal reagent from Antibodies.com detects the protein in human, mouse, and rat samples. Validated for Western blot and immunohistochemistry, the antibody is supplied as 100 µL of antiserum and supports studies of V-ATPase–driven acidification in endosomes, lysosomes, and secretory vesicles. The polyclonal format broadens epitope coverage, helping researchers pick up multiple regions of the target even when expression is modest or post-translational modification differs between sample types.

Working dilutions should be titrated empirically; common Western blot applications begin in the 1:500–1:2000 range with chemiluminescent detection, while immunohistochemistry on paraffin-embedded sections is frequently performed at 1:100–1:500 following antigen retrieval. Because ATP6AP1 is a multi-pass transmembrane component of the V-ATPase complex, membrane-enriched fractions or fixed tissue sections often yield cleaner signals than whole-lysate blots. Store the vial refrigerated at 4 °C for short-term use and aliquot before freezing for longer-term storage to minimize freeze-thaw cycles.