Anti-ATP5B Antibody

Mitochondrial energy production depends on ATP synthase, a multi-subunit enzyme complex embedded in the inner mitochondrial membrane. The beta subunit, encoded by ATP5B, forms the catalytic core responsible for synthesizing ATP from ADP and inorganic phosphate using the proton gradient. This unconjugated rabbit polyclonal antibody from Antibodies.com specifically targets ATP5B and is supplied as 100 µl. Validated for Western blot (WB) and immunohistochemistry (IHC), it shows broad species reactivity against human, mouse, and rat samples, supporting comparative studies across model organisms. Researchers use ATP5B detection for investigations into oxidative phosphorylation efficiency, mitochondrial biogenesis, metabolic disease mechanisms, and as a mitochondrial loading control during cell lysate normalization. The polyclonal antibody format enables recognition of multiple epitopes on the target, providing robust signal amplification in both immunoblotting and tissue staining workflows.