Anti-ATP5A Antibody

Raised in rabbit against the alpha subunit of mitochondrial ATP synthase, this polyclonal primary antibody from Antibodies.com targets a catalytic core component of the F1 complex that couples proton motive force to ATP synthesis during oxidative phosphorylation. Supplied as an unconjugated IgG preparation at 100 µl per vial, the reagent recognizes a conserved epitope shared across human, mouse, and rat orthologs.

Validated for western blot, immunohistochemistry, and ELISA workflows, the antibody serves as a robust marker in studies of mitochondrial biology. On immunoblots it functions as a mitochondrial loading control alongside cytoskeletal or housekeeping reference proteins, because ATP5A abundance reflects intact mitochondrial mass rather than transcriptional regulation alone. In immunohistochemistry on formaldehyde-fixed, paraffin-embedded tissue sections, the polyclonal reagent produces granular cytoplasmic staining consistent with mitochondrial distribution; the polyclonal format increases tolerance to minor epitope modifications introduced by formalin crosslinking, which often helps recover signal in archived material.

Standard antibody handling applies: aliquot on arrival to limit freeze-thaw cycles, store frozen for long-term use, and keep working stocks refrigerated. Researchers investigating mitochondrial biogenesis, electron transport chain assembly, neurodegeneration models, and metabolic disease phenotypes will find this Antibodies.com reagent applicable across immunoblot, immunostaining, and quantitative immunoassay readouts, supporting both routine phenotyping and mechanistic dissection of cellular energy metabolism.