Anti-ATP synthase subunit delta Antibody

Raised in rabbit against the mitochondrial delta subunit of ATP synthase (also called Complex V), this unconjugated polyclonal antibody recognizes the human protein encoded by ATP5F1D and is suited to studies of oxidative phosphorylation, respiratory chain assembly, and mitochondrial bioenergetics. Each vial contains 100 µg of IgG supplied as a liquid preparation suitable for western blot analysis of the delta subunit in cell and tissue lysates and for immunohistochemistry on formalin-fixed, paraffin-embedded sections. The reagent detects the endogenous human antigen, a peripheral stalk component that mechanically couples F1 catalytic activity to F0 proton translocation across the inner mitochondrial membrane. Antibodies.com includes this reagent in its primary antibody catalogue; storing the vial frozen at -20 °C in single-use aliquots preserves binding activity through repeated freeze-thaw cycles, and working dilutions should be titrated empirically for chemiluminescent western blot imaging or for peroxidase- and fluorescence-based IHC workflows. Researchers profiling Complex V content in models of neurodegeneration, cardiac ischemia-reperfusion injury, and tumor metabolic reprogramming commonly pair this antibody with mitochondrial loading controls and OXPHOS complex antibodies for subunit-resolved respiratory chain analysis.