Anti-ATM Antibody

ATM (Ataxia-Telangiectasia Mutated) is a serine/threonine protein kinase central to the cellular DNA damage response. This recombinant rabbit monoclonal antibody from Antibodies.com provides a highly specific reagent for detecting endogenous ATM protein across multiple experimental platforms, including Western blot, immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC). Raised in rabbit and validated for human reactivity, the unconjugated antibody is supplied in a 50 µl vial in TBS buffer (pH 7.4) containing 1% BSA, 40% glycerol, and 0.05% sodium azide as preservative, allowing direct conjugation or pairing with secondary reagents of the researcher's choice.

ATM kinase is recruited to DNA double-strand breaks, where it phosphorylates downstream effectors such as p53, Chk2, BRCA1, and H2AX, making reliable detection of total ATM levels essential for studies of genome stability, cell cycle checkpoint control, neurodegeneration, radiosensitivity, and tumor biology. Researchers use this reagent to characterize ATM expression in patient-derived samples, cell lines exposed to genotoxic agents, and tissue sections from cancer or ataxia-telangiectasia models.