Anti-ATG5 Antibody

Affinity-purified and unconjugated, this Rabbit polyclonal reagent from Antibodies.com specifically recognizes ATG5 (autophagy-related protein 5) and is supplied at a working concentration of 1.0 mg/mL in a 50 µL volume. Raised against a recombinant human ATG5 immunogen, the antibody detects the endogenous total ATG5 protein and has been validated for Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry (IHC). Reactivity has been confirmed in human, mouse, and rat samples, supporting autophagy research across the most commonly used mammalian model systems. The product is formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, supplemented with 150 mM NaCl, 0.02% sodium azide as a preservative, and 50% glycerol as a cryoprotectant. According to the Antibodies.com shipping and storage guidelines, the vial is shipped at 4 °C; upon receipt, it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve binding activity and reagent stability. Polyclonal host species (Rabbit), isotype-equivalent IgG fraction purified by antigen-affinity chromatography, and unconjugated status make this reagent compatible with a broad range of secondary antibody detection chemistries. For research use only — not intended for diagnostic or therapeutic applications.