Anti-ATG5 Antibody

ATG5 is a central regulator of macroautophagy, best known as a core component of the ATG5–ATG12 conjugate that drives autophagosome membrane elongation and LC3 lipidation. This unconjugated rabbit polyclonal reagent from Antibodies.com recognizes endogenous ATG5 and the higher-molecular-weight ATG5–ATG12 complex in human and mouse samples, supporting autophagy research across cell lines, primary cultures, and tissue specimens.

The antibody is supplied as 100 µl of polyclonal antiserum, validated for Western blot detection of the ~32 kDa ATG5 band alongside the ~56 kDa ATG5–ATG12 conjugate, and for immunohistochemistry on formalin-fixed paraffin-embedded material. Staining of human and mouse targets is supported by the manufacturer; predicted reactivity with rat by sequence homology should be confirmed in-house before use.

Researchers commonly apply this reagent to monitor autophagic flux, study the interplay between autophagy and apoptosis via calpain-mediated ATG5 cleavage, and investigate ATG5 function in cancer, neurodegeneration, infection biology, and metabolic disease models.