Anti-ATG4C Antibody

ATG4C (Autophagy Related 4C Cysteine Peptidase) is a cysteine protease that plays a central role in the autophagy machinery by cleaving pro-LC3 and other ATG8 family members to generate their lipid-conjugated, autophagosome-associated forms. This unconjugated rabbit polyclonal antibody, supplied by Antibodies.com, is raised against ATG4C and validated for Western blot and immunohistochemistry workflows.

The antibody is provided at 1 mg/ml in a PBS buffer containing 0.1% sodium azide and 50% glycerol, and is reactive against human, mouse, and rat samples. Each vial contains 100 µl of antibody solution. Researchers investigating autophagy regulation, mitophagy, or the ATG8 conjugation system can use this reagent to probe endogenous ATG4C expression across diverse cell lines and tissue types, with consistent performance in both denaturing immunoblotting and fixed-tissue IHC protocols.

The polyclonal format recognizes multiple epitopes on ATG4C, which is helpful when studying processing intermediates or partial proteolysis products. Western blot applications typically resolve a band near 52 kDa, while immunohistochemistry enables cellular localization studies in formalin-fixed tissue sections.