Anti-ATG10 Antibody

Targeting Autophagy Related 10 (ATG10), the E2-like conjugating enzyme that pairs with ATG7 to attach ATG12 onto ATG5 during autophagosome biogenesis, this rabbit polyclonal primary antibody from Antibodies.com is supplied unconjugated in a 100 µl vial. It supports research into the ubiquitin-like conjugation cascade that drives macroautophagy in eukaryotic cells.

Validated for western blot and immunohistochemistry, the reagent detects endogenous ATG10 across both human and mouse samples, enabling comparative studies of basal autophagy, stress-induced autophagic flux, and mitophagy models. The polyclonal rabbit format increases the likelihood of recognizing multiple epitopes on the target, while the unconjugated presentation lets researchers pair it with secondary reagents of their choice for chromogenic, fluorescent, or chemiluminescent readout.

For western blot, the antibody is applied to whole-cell or tissue lysates to monitor ATG10 expression under different experimental conditions. For immunohistochemistry, it suits formalin-fixed, paraffin-embedded sections for spatial mapping of ATG10 distribution within tissues. Aliquot the reagent upon arrival, refrigerate, and protect from light; avoid repeated freeze-thaw cycles to preserve performance for research use only.