Anti-ATF6 Antibody

This rabbit polyclonal antibody from Antibodies.com targets ATF6 (Activating Transcription Factor 6), a type II transmembrane transcription factor anchored in the endoplasmic reticulum membrane that acts as a central sensor of the unfolded protein response (UPR). During accumulation of misfolded proteins, ATF6 translocates to the Golgi apparatus where site-1 and site-2 proteases cleave its cytosolic bZIP domain; the liberated fragment enters the nucleus and upregulates ER-resident chaperones including BiP/GRP78 alongside XBP1.

The reagent is supplied in a single vial containing 100 µl of polyclonal antiserum at 1 mg/ml, formulated in PBS with 0.1% sodium azide and 50% glycerol for storage stability. Researchers can apply it to western blot, immunohistochemistry, and immunofluorescence workflows using human, mouse, or rat samples, supporting studies of ER stress in cell culture and fixed tissue sections. Unconjugated presentation permits pairing with HRP- or fluorophore-labeled secondary reagents as required by the chosen detection method. Store aliquots at -20 °C and avoid repeated freeze-thaw cycles to preserve antibody activity.