Anti-ATF4 (phospho S245) Antibody

Produced in rabbit as a polyclonal IgG and supplied unconjugated at 200 µl, this Antibodies.com antibody specifically recognises the activating transcription factor ATF4 only when phosphorylated on serine 245, the modification that marks its activated form during the integrated stress response and endoplasmic reticulum stress signaling. It is reactive with human samples and has been validated for Western blot work on cell lysates and for immunohistochemistry on fixed tissue sections, giving researchers a way to monitor stress-pathway activation biochemically and observe its spatial distribution in tissue. The polyclonal format provides broad epitope coverage around the phospho-site, helping detection in samples where modified residues vary between conditions, and the unconjugated rabbit IgG backbone accommodates any compatible secondary antibody and detection chemistry. Groups studying tumor hypoxia, neurodegenerative protein-misfolding disease, or pharmacological engagement of the PERK and GCN2 arms of the ISR may use this reagent to track pathway activity downstream of eIF2α phosphorylation. Aliquot upon arrival, avoid repeated freeze-thaw cycles, and protect from light to preserve antibody performance across multiple experiments and blots.