Anti-ATF4 Antibody

This rabbit polyclonal antibody targets ATF4 (Activating Transcription Factor 4), a stress-responsive leucine zipper transcription factor central to the integrated stress response, unfolded protein response, and amino acid deprivation pathways. Supplied by Antibodies.com as a 50 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated reagent is validated for Western blot (WB) and immunohistochemistry (IHC) applications across Human, Mouse, and Rat samples.

ATF4 acts as a master effector downstream of PERK-eIF2α signaling, where it binds the C/EBP-ATF composite (CARE) element to drive expression of genes involved in amino acid metabolism, autophagy, antioxidant defenses, and osteoblast biology. The polyclonal reagent is widely used to investigate ER-stress pathways in cancer models, neurodegeneration studies, and research on metabolic adaptation.

For handling, store the vial at -20°C and dispense into small aliquots to minimize freeze-thaw cycles; the 50% glycerol cryoprotectant preserves antibody integrity during routine use. A brief centrifugation step before opening recovers any liquid clinging to the cap or vial walls.