Anti-ATF4 Antibody

Supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, this unconjugated rabbit polyclonal antibody from Antibodies.com targets ATF4 (Activating Transcription Factor 4), a basic leucine zipper transcription factor central to the integrated stress response, amino acid metabolism, and the unfolded protein response (UPR) pathways triggered by ER stress and eIF2α phosphorylation.

Validated applications include Western blot and immunohistochemistry on human, mouse, and rat samples, supporting expression and localization studies in whole-cell lysates, subcellular fractions, and formalin-fixed tissue sections. The unconjugated format leaves labeling choice open, allowing pairing with anti-rabbit HRP, fluorophore-conjugated secondary reagents, or biotin-streptavidin amplification depending on the experimental design and detection platform.

Store at -20 °C for long-term preservation; aliquot upon first thaw to limit freeze-thaw cycles, and protect working dilutions from prolonged light exposure. Sodium azide is present at 0.1% as a preservative and should be accounted for in any downstream live-cell or in vivo conjugation workflow.