Anti-ATF2 (phospho T73 + T55) Antibody

Stress-activated kinases such as JNK and p38 MAPK target ATF2 at threonine 55 and threonine 73, and this rabbit polyclonal IgG reagent from Antibodies.com provides a phospho-specific means of monitoring that dual modification in cell and tissue samples. The antibody was raised against a synthetic phospho-peptide spanning residues 40–89 of human ATF2 around the T-P-T(p)-R-F motif and is affinity-purified against the phosphorylated epitope, with non-phospho-specific immunoglobulins removed by sequential chromatography on non-phospho-peptide columns. It detects endogenous ATF2 only when phosphorylated at Thr73 or Thr55, making it a useful reagent for studies of DNA damage response, inflammatory signaling, osmotic stress, and UV- or anisomycin-induced activation in cultured cells and tissue sections.

Validated in western blot at 1:500–1:1000, immunohistochemistry at 1:50–1:100, and immunofluorescence at 1:100–1:500, the unconjugated antibody reacts with human, mouse, and rat ATF2 and yields a band near the expected 52 kDa molecular weight (commonly observed between 55 and 75 kDa owing to post-translational modification). Each vial provides 200 µl of liquid antibody formulated in PBS pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant; aliquoting upon receipt and limiting freeze-thaw cycles helps preserve phospho-specific binding activity across multiple experiments. For long-term storage, keep the vial frozen at -20°C and protect from light to safeguard antibody performance over time.