Anti-ATF2 (phospho T71 + T53) Antibody

Raised in rabbit and affinity-purified against a synthetic phosphopeptide spanning residues 38–87 of human ATF2 around threonine 71 and threonine 53, this unconjugated polyclonal IgG reagent from Antibodies.com selectively recognizes the transcription factor only in its phosphorylated state, sparing unphosphorylated ATF2. Because Thr71 and Thr53 are direct substrates of stress-activated kinases including p38, JNK, and ERK, the antibody is commonly used to track activation of ATF2-driven transcriptional programs tied to UV and genotoxic stress, growth-factor signaling, chromatin remodeling via intrinsic HAT activity, and DNA-damage responses.

Validated uses cover Western blot at 1:500–1:1000 and immunohistochemistry on formalin-fixed paraffin-embedded sections at 1:50–1:100, with reactivity confirmed in human, mouse, and rat samples. The 50 µl liquid aliquot is formulated in PBS (pH 7.4) with 50% glycerol and 0.02% sodium azide; store at -20°C and avoid repeated freeze-thaw cycles to preserve phospho-specific binding. For immunoblotting, dilute in 5% BSA, 1X TBS, 0.1% Tween-20 and incubate the membrane overnight at 4°C with gentle shaking, then pair with an HRP- or fluorophore-conjugated secondary matched to the chosen detection system.