Anti-ATF1 Antibody

Manufactured by Antibodies.com as a high-quality research reagent, this affinity-purified rabbit polyclonal antibody is raised against a synthetic peptide derived from the internal region of human ATF1 (cyclic AMP-dependent transcription factor ATF-1), enabling sensitive detection of endogenous total ATF1 protein in a broad range of experimental systems. The antibody is supplied as a 50 µl aliquot at a working concentration of 1.0 mg/mL and is unconjugated, allowing flexibility for downstream labelling. It is formulated in rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, with 150 mM NaCl, 0.02% sodium azide and 50% glycerol. Validated applications include Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF), and reactivity has been confirmed in Human, Mouse, and Rat samples. The polyclonal host species is rabbit, providing recognition of multiple epitopes on ATF1 for robust signal. For best stability, the product is shipped at 4°C and should be aliquoted upon delivery and stored at -20°C, with freeze/thaw cycles avoided to preserve activity. Each lot is affinity-purified from rabbit antiserum by affinity chromatography using the epitope-specific immunogen, supporting lot-to-lot consistency for routine signalling and transcription factor studies.