Anti-ASMT Antibody

ASMT (acetylserotonin O-methyltransferase) catalyzes the terminal step of melatonin biosynthesis in the pineal gland, transferring a methyl group from S-adenosylmethionine to N-acetylserotonin to yield melatonin. The Antibodies.com rabbit polyclonal reagent targets the human ASMT protein and is supplied as 100 µl of unconjugated antiserum, giving laboratories flexibility to choose their own secondary detection chemistry.

Validated workflows include Western blot for protein-level analysis in cell or tissue lysates and immunofluorescence for visualizing ASMT localization in fixed sections. The unconjugated format pairs cleanly with HRP-, fluorophore-, or biotin-conjugated secondary reagents selected by the researcher, accommodating both chemiluminescent imaging and confocal or widefield microscopy.

Polyclonal preparations recognize multiple epitopes across the target, which can be helpful when studying tissue-specific expression patterns in human pineal and retinal samples, where ASMT contributes to local melatonin production. The antibody is intended for research use only and supports investigation into circadian biology, pineal gland function, and the serotonin-to-melatonin signaling pathway.