Anti-ASK1 (V972) Antibody

This polyclonal antibody from Antibodies.com targets apoptosis signal-regulating kinase 1 (ASK1), also known as MAP3K5, a stress-activated MAP kinase kinase kinase that sits upstream of the JNK and p38 MAPK cascades. Raised in rabbit against a region centered on residue V972 of the human protein, the reagent is supplied as 100 µL of a 1 mg/ml stock in phosphate-buffered saline with 0.05% sodium azide at approximately pH 7.2.

The antibody is unconjugated and validated for immunohistochemistry on formalin-fixed tissue sections from human, mouse, and rat samples, where it is used to map ASK1 expression in stressed or inflamed tissue. Because ASK1 is activated by oxidative stress, endoplasmic reticulum stress, TNF cytokine signaling, and intracellular calcium overload, the reagent is applied in cardiovascular injury, neurodegeneration, kidney damage, and cytokine-driven inflammation models, where investigators compare staining intensity across treated versus control cohorts.

Working aliquots should be refrigerated and protected from light, with the sodium azide preservative allowing short-term handling at room temperature without microbial contamination. For multiplexing, the unconjugated format pairs cleanly with enzyme- or fluorophore-labeled secondary antibodies chosen by the end user, including anti-rabbit IgG reagents compatible with chromogenic DAB or immunofluorescence detection.