Anti-ASK1 (phospho-T918) Antibody

A phospho-specific polyclonal reagent from Antibodies.com raised in rabbit and affinity-purified to recognize ASK1 (MAP3K5) only when threonine 918 is phosphorylated. Threonine 918 lies within the activation loop of this stress-responsive kinase, and phosphorylation at this residue serves as a widely used readout of ASK1 activation within MAPK cascade signalling that proceeds through the JNK and p38 pathways during oxidative stress, ER stress, and inflammatory cytokine responses in cellular models.

Validated for Western blot on Human, Mouse, and Rat samples, the unconjugated reagent is supplied at 1 mg/mL in PBS containing 0.1% sodium azide and 50% glycerol, packaged as a 100 µL aliquot. For best stability, store at -20 °C, divide into single-use aliquots to limit freeze-thaw cycles, and protect from light between uses. Signal is observed at the expected ~155 kDa band corresponding to full-length ASK1, with phospho-specificity that can be confirmed by treating lysates with lambda phosphatase prior to immunoblotting.

Used as a primary antibody in cell biology research on apoptosis regulation, cytokine signalling, and stress-activated kinase pathways, this rabbit polyclonal reagent supports immunoblotting workflows across Human, Mouse, and Rat reactivity panels.