Anti-ASK1 (Ab-966) Antibody

This polyclonal antibody from Antibodies.com selectively recognizes ASK1 (Apoptosis Signal-regulating Kinase 1, also called MAP3K5) when phosphorylated at threonine 966, a key activation-loop autophosphorylation site that marks the kinase-active form of the enzyme. Raised in rabbit, the reagent is supplied as 100 µL of unconjugated antiserum validated for Western blot, immunohistochemistry, and immunofluorescence workflows across human, mouse, and rat samples.

Because the epitope centers on phospho-Thr966 rather than total ASK1 protein, this reagent supports studies of stress-activated kinase signaling downstream of TNF, oxidative, and endoplasmic reticulum challenge, where the appearance of a slower-migrating phospho-ASK1 band on immunoblots is a widely used readout of pathway engagement. The polyclonal format raises the likelihood of detecting ASK1 even when target expression is modest, while the unconjugated presentation allows laboratories to pair the primary with their preferred secondary detection systems, fluorophores, or enzyme labels.

For handling, aliquot on receipt and store refrigerated, avoiding repeated freeze-thaw cycles that erode affinity for the phosphoepitope and compromise signal in downstream applications.